SidaGhanaV1, Main, Exploration, bibRecord, 000232

Bacterial etiology of sexually transmitted infections at a STI clinic in Ghana; use of multiplex real time PCR

Identifieur interne : 000232 ( Main/Exploration ); précédent : 000231; suivant : 000233

Bacterial etiology of sexually transmitted infections at a STI clinic in Ghana; use of multiplex real time PCR

Auteurs : Augustina A. Sylverken [Ghana] ; Ellis Owusu-Dabo [Ghana] ; Denis D. Yar [Ghana] ; Samson P. Salifu [Ghana] ; Nana Yaa Awua-Boateng [Ghana] ; John H. Amuasi [Ghana] ; Portia B. Okyere [Ghana] ; Thomas Agyarko-Poku [Ghana]

Source :

Ghana Medical Journal [ 0016-9560 ] ; 2016.

RBID : PMC:5044789

Abstract

SummaryBackground

Most sexually transmitted infection (STI) management efforts focus on the syndromic approach to diagnose and treat patients. However, most women with STIs have been shown to be entirely asymptomatic, or if symptoms exist, are often missed when either clinical or conventional bacteriologic diagnostic tools are employed.

Methods

We assessed the performance of a multiplex real time PCR assay to describe other potential pathogens that could be missed by conventional bacteriological techniques in 200 women attending a routine STI clinic in Kumasi, Ghana.

Results

Although a total 78.00% of the women were asymptomatic, 77.1% of them tested positive for at least one bacterial STI pathogen. Mycoplasma genitalium was the most commonly detectable pathogen present in 67.5% of all women. Of those testing positive, 25.0% had single infections, while 38.0% and 19.5% had double and triple infections respectively. Altogether, 86.54% and 90.91% of the symptomatic and asymptomatic women respectively tested positive for at least one pathogen (p<0.05). There were no significant associations (p<0.05) between the clinical manifestations of the symptomatic women and the pathogens detected in their samples.

Conclusions

Our study confirmed the importance of complementing the syndromic approach to STI management with pathogen detection and most importantly recognise that STIs in women are asymptomatic and regular empirical testing even for both symptomatic and asymptomatic patients is critical for complete clinical treatment.

Funding

EOD (Ellis Owusu-Dabo Research working group, KCCR)

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5044789

PubMed: 27752188
PubMed Central: 5044789

Affiliations:

Ghana

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<front><div type="abstract" xml:lang="en"><title>Summary</title>
<sec><title>Background</title>
<p>Most sexually transmitted infection (STI) management efforts focus on the syndromic approach to diagnose and treat patients. However, most women with STIs have been shown to be entirely asymptomatic, or if symptoms exist, are often missed when either clinical or conventional bacteriologic diagnostic tools are employed.</p>
</sec>
<sec sec-type="methods"><title>Methods</title>
<p>We assessed the performance of a multiplex real time PCR assay to describe other potential pathogens that could be missed by conventional bacteriological techniques in 200 women attending a routine STI clinic in Kumasi, Ghana.</p>
</sec>
<sec sec-type="results"><title>Results</title>
<p>Although a total 78.00% of the women were asymptomatic, 77.1% of them tested positive for at least one bacterial STI pathogen. <italic>Mycoplasma genitalium</italic>
 was the most commonly detectable pathogen present in 67.5% of all women. Of those testing positive, 25.0% had single infections, while 38.0% and 19.5% had double and triple infections respectively. Altogether, 86.54% and 90.91% of the symptomatic and asymptomatic women respectively tested positive for at least one pathogen (p<0.05). There were no significant associations (p<0.05) between the clinical manifestations of the symptomatic women and the pathogens detected in their samples.</p>
</sec>
<sec sec-type="conclusions"><title>Conclusions</title>
<p>Our study confirmed the importance of complementing the syndromic approach to STI management with pathogen detection and most importantly recognise that STIs in women are asymptomatic and regular empirical testing even for both symptomatic and asymptomatic patients is critical for complete clinical treatment.</p>
</sec>
<sec><title>Funding</title>
<p><bold>EOD (Ellis Owusu-Dabo Research working group, KCCR)</bold>
</p>
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Le SIDA au Ghana (serveur d'exploration)

Bacterial etiology of sexually transmitted infections at a STI clinic in Ghana; use of multiplex real time PCR

Bacterial etiology of sexually transmitted infections at a STI clinic in Ghana; use of multiplex real time PCR

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